Binding of tris(2,2′−dimethoxy−2,2′−bipyriyl) ruthenium (II) complex with quinones have been studied in 50% aqueous acetonitrile medium by means of absorption spectral technique. The absorption and emission maximum of [Ru(dmeObpy)3]²⁺ complex are 468 nm and 606 nm respectively. The binding constants for the reactions of the complex with quinones are determined using the Benesi-Hildebrand plot using the absorption intensity data. The value of Kb is sensitive to the structure and nature of the quenchers used. Structural effect also plays a significant role on the binding of the complex with quinones.